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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP11B2 Polyclonal antibody (20968-1-AP)

    WB analysis using 20968-1-AP (same clone as 20968-1-PBS)

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using CYP11B2 Polyclonal antibody (20968-1-AP)

    WB analysis of SKOV-3 using 20968-1-AP (same clone as 20968-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human adrenal gland tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human adrenal gland using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human adrenal gland tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human adrenal gland using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human kidney using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human kidney using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human hepatocirrhosis using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human hepatocirrhosis using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human liver cancer using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human liver cancer using 20968-1-AP (same clone as 20968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using CYP11B2 Polyclonal antibody (20968-1-AP)

    IF Staining of SKOV-3 using 20968-1-AP (same clone as 20968-1-PBS)

    Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20968-1-PBS in a different storage buffer formulation.

CYP11B2 Polyclonal antibody, PBS Only

CYP11B2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20968-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20968-1-PBS targets CYP11B2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 11, subfamily B, polypeptide 2
Calculated molecular weight 58 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberNM_000498
Gene Symbol CYP11B2
Gene ID (NCBI) 1585
RRIDAB_2878777
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19099
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

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