Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, HepG2 cells, SiHa cells, HeLa cells, Caco-2 cells. human placenta tissue, human testis tissue, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human placenta tissue, rat adrenal gland tissue, mouse adrenal gland tissue, human testis tissue, mouse testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
67264-1-Ig targets CYP11A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4353 Product name: Recombinant human CYP11A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC032329 Sequence: MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRG 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 11, subfamily A, polypeptide 1 |
| Calculated molecular weight | 521 aa, 60 kDa |
| Observed molecular weight | 49-52 kDa |
| GenBank accession number | BC032329 |
| Gene Symbol | CYP11A1 |
| Gene ID (NCBI) | 1583 |
| RRID | AB_2882535 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05108 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| WB protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Pollut Arsenic exposure reduces testosterone synthesis partially by evoking Leydig cell ferroptosis in mouse testes. | ||
J Ethnopharmacol Saffron extract alleviates D-gal-induced late-onset hypogonadism by activating the PI3K-Akt-Nrf2 signaling pathway | ||
Ecotoxicol Environ Saf Gene-environment interaction in hypospadias: DEHP exposure aggravates DNAH8-related disruption of testicular steroidogenesis. | ||
Int Immunopharmacol Melatonin orchestrates cAMP-PKA signaling to alleviate zearalenone-impaired the estradiol synthesis in mammal ovary. | ||
Mol Hum Reprod Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction |