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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CYB5R4 Polyclonal antibody (12626-1-AP)

    WB analysis of BxPC-3 using 12626-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CYB5R4 Polyclonal antibody (12626-1-AP)

    WB analysis using 12626-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using CYB5R4 Polyclonal antibody (12626-1-AP)

    WB analysis of C6 using 12626-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CYB5R4 Polyclonal antibody (12626-1-AP)

    WB analysis of HeLa using 12626-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CYB5R4 Polyclonal antibody (12626-1-AP)

    IHC staining of mouse kidney using 12626-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12626-1-AP (CYB5R4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CYB5R4 Polyclonal antibody (12626-1-AP)

    IHC staining of mouse kidney using 12626-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12626-1-AP (CYB5R4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYB5R4 Polyclonal antibody

CYB5R4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12626-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

b5+b5R, cb5/cb5R, Cytochrome b5 reductase 4, EC:1.6.2.2, Flavohemoprotein b5/b5R

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inIMR-32 cells, HeLa cells, BxPC-3 cells, C6 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12626-1-AP targets CYB5R4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3318

Product name: Recombinant human CYB5R4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 220-521 aa of BC025380

Sequence: GLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQLISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYFLIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLRKVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA

相同性解析による交差性が予測される生物種
Full Name cytochrome b5 reductase 4
Calculated molecular weight55.8 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC025380
Gene Symbol CYB5R4
Gene ID (NCBI) 51167
RRIDAB_2090295
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L1T6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CYB5R4 antibody 12626-1-APDownload protocol
WB protocol for CYB5R4 antibody 12626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Redox Biol

Cytochrome b5 reductase is the component from neuronal synaptic plasma membrane vesicles that generates superoxide anion upon stimulation by cytochrome c.

Authors - Alejandro K Samhan-Arias
View Article
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