• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

9C4D10

Cat no : 66022-1-Ig

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66022-1-Ig

    WB analysis of HeLa using 66022-1-Ig

    WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells.

  • WB analysis of HeLa using 66022-1-Ig

    WB analysis of HeLa using 66022-1-Ig

    Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 66022-1-Ig

    WB analysis of human plasma using 66022-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 66022-1-Ig

    WB analysis of L02 using 66022-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66022-1-Ig

    WB analysis of HeLa using 66022-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 66022-1-Ig

    WB analysis of human heart using 66022-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66022-1-Ig

    WB analysis of HepG2 using 66022-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 66022-1-Ig

    WB analysis of human heart using 66022-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66022-1-Ig

    IHC staining of human liver cancer using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human liver cancer using 66022-1-Ig

    IHC staining of human liver cancer using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human liver using 66022-1-Ig

    IHC staining of human liver using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human liver using 66022-1-Ig

    IHC staining of human liver using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human placenta using 66022-1-Ig

    IHC staining of human placenta using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IHC staining of human placenta using 66022-1-Ig

    IHC staining of human placenta using 66022-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CYB5R3 antibody included
  • IF Staining of HeLa using 66022-1-Ig

    IF Staining of HeLa using 66022-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 66022-1-Ig

    IF Staining of HeLa using 66022-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 66022-1-Ig

    IF Staining of HepG2 using 66022-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human heart tissue, human plasma tissue, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66022-1-Ig targets CYB5R3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag18096 相同性解析による交差性が予測される生物種
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC004821
Gene symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_11045662
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
WB protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IHC protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IF protocol for CYB5R3 antibody 66022-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols