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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    WB analysis of human liver using 12365-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12365-1-AP (CYB5A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human pancreas cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12365-1-AP (CYB5A antibody) at dilution of 1:50 (under 10x lens).

Cytochrome b5 Polyclonal antibody

Cytochrome b5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12365-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CYB5, CYB5A, Cytochrome b5, MCB5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12365-1-AP targets Cytochrome b5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3038

Product name: Recombinant human Cytochrome b5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-108 aa of BC015182

Sequence: MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSS

相同性解析による交差性が予測される生物種
Full Name cytochrome b5 type A (microsomal)
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC015182
Gene Symbol Cytochrome b5
Gene ID (NCBI) 1528
RRIDAB_2089808
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP00167
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Cytochrome b5 antibody 12365-1-APDownload protocol
WB protocol for Cytochrome b5 antibody 12365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics Clin Appl

Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.

Authors - Yueyan Li
View Article
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