• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human liver tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    WB analysis of human liver using 12365-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12365-1-AP (CYB5A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human pancreas cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12365-1-AP (CYB5A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome b5 Polyclonal antibody (12365-1-AP)

    IHC staining of human liver cancer using 12365-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12365-1-AP (Cytochrome b5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Cytochrome b5 Polyclonal antibody

Cytochrome b5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12365-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CYB5, CYB5A, Cytochrome b5, MCB5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1200 ug/ml
20 μL, 1200 ug/ml
150 μL, 1200 ug/ml

¥67,000/ 150 μL

本製品の国内在庫をすぐに見る（表示に数秒かかります）

【PR】一次抗体まとめて特別価格！『PRIME BUY抗体特割キャンペーン』4/21スタート

他社製品の代替品をお探しですか？『抗体代替品／同等品検索システム』でお調べいただけます

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12365-1-AP targets Cytochrome b5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytochrome b5 fusion protein Ag3038 相同性解析による交差性が予測される生物種
Full Name cytochrome b5 type A (microsomal)
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC015182
Gene Symbol Cytochrome b5
Gene ID (NCBI) 1528
RRIDAB_2089808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00167
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cytochrome b5 antibody 12365-1-APDownload protocol
IHC protocol for Cytochrome b5 antibody 12365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics Clin Appl

Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.

Authors - Yueyan Li
View Article