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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CXorf56 Polyclonal antibody (24021-1-AP)

    WB analysis of HeLa using 24021-1-AP (same clone as 24021-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CXorf56 Polyclonal antibody (24021-1-AP)

    WB analysis of mouse kidney using 24021-1-AP (same clone as 24021-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CXorf56 Polyclonal antibody (24021-1-AP)

    WB analysis of HEK-293 using 24021-1-AP (same clone as 24021-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24021-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using CXorf56 Polyclonal antibody (24021-1-AP)

    IP experiment of mouse kidney using 24021-1-AP (same clone as 24021-1-PBS)

    IP result of anti-CXorf56 (IP:24021-1-AP, 4ug; Detection:24021-1-AP 1:1000) with mouse kidney tissue lysate 8000ug. This data was developed using the same antibody clone with 24021-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CXorf56 Polyclonal antibody (24021-1-AP)

    IF Staining of HeLa using 24021-1-AP (same clone as 24021-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24021-1-AP (CXorf56 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24021-1-PBS in a different storage buffer formulation.

CXorf56 Polyclonal antibody, PBS Only

CXorf56 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 24021-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24021-1-PBS targets CXorf56 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19856

Product name: Recombinant human CXorf56 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-222 aa of BC023506

Sequence: MPKVVSRSVVCSDTRDREEYDDGEKPLHVYYCLCGQMVLVLDCQLEKLPMRPRDRSRVIDAAKHAHKFCNTEDEETMYLRRPEGIERQYRKKCAKCGLPLFYQSQPKNAPVTFIVDGAVVKFGQGFGKTNIYTQKQEPPKKVMMTKRTKDMGKFSSVTVSTIDEEEEEIEAREVADSYAQNAKVIEKQLERKGMSKRRLQELAELEAKKAKMKGTLIDNQFK

相同性解析による交差性が予測される生物種
Full Name chromosome X open reading frame 56
Calculated molecular weight 222 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC023506
Gene Symbol CXorf56
Gene ID (NCBI) 63932
RRIDAB_2879404
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H5V9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CxORF56 (also known as STEEP) is an evolutionarily highly conserved protein whose function is unknown. Recently CxORF56 has been identified as a protein that interacts with STING, and it is essential for STING ER exit. CxORF56-dependent STING ER exit is essential for STING signaling in antiviral defense and in inflammatory diseases.

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