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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    WB analysis of mouse lung using 20837-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20837-1-AP (CXorf22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    IHC staining of human lung cancer using 20837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    IHC staining of human lung cancer using 20837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    IHC staining of human lung using 20837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    IHC staining of human lung using 20837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    IHC staining of human spleen using 20837-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CXorf22 Polyclonal antibody (20837-1-AP)

    IHC staining of human spleen using 20837-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CFAP47 Polyclonal antibody (20837-1-AP)

    IF Staining of mouse kidney using 20837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CFAP47 antibody (20837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CFAP47 Polyclonal antibody

CFAP47 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20837-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

CXorf22, CHDC2, Cilia- and flagella-associated protein 47, CXorf30, CXorf59

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue, human lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20837-1-AP targets CFAP47 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFAP47 fusion protein Ag14910 相同性解析による交差性が予測される生物種
Full Name chromosome X open reading frame 22
Calculated molecular weight 976 aa, 110 kDa
GenBank accession numberBC027936
Gene Symbol CFAP47/CXorf22
Gene ID (NCBI) 170063
RRIDAB_11232036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZTR5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CFAP47 antibody 20837-1-APDownload protocol
IHC protocol for CFAP47 antibody 20837-1-APDownload protocol
IF protocol for CFAP47 antibody 20837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF,CoIP

Mol Hum Reprod

WDR87 interacts with CFAP47 protein in the middle piece of spermatozoa flagella to participate in sperm tail assembly

Authors - Hong-Qing Liao
View Article
humanIF

medRxiv

CFAP47 is a novel causative gene implicated in X-linked polycystic kidney disease

Authors - Takayasu Mori
View Article