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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXorf15 Polyclonal antibody (22357-1-AP)

    WB analysis using 22357-1-AP (same clone as 22357-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CXorf15 Polyclonal antibody (22357-1-AP)

    IHC staining of mouse heart using 22357-1-AP (same clone as 22357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22357-1-AP (CXorf15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CXorf15 Polyclonal antibody (22357-1-AP)

    IHC staining of mouse heart using 22357-1-AP (same clone as 22357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22357-1-AP (CXorf15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CXorf15 Polyclonal antibody (22357-1-AP)

    IHC staining of human ovary cancer using 22357-1-AP (same clone as 22357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22357-1-AP (CXorf15 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22357-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CXorf15 Polyclonal antibody (22357-1-AP)

    IF Staining of HEK-293 using 22357-1-AP (same clone as 22357-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 22357-1-AP (CXorf15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22357-1-PBS in a different storage buffer formulation.

CXorf15 Polyclonal antibody, PBS Only

CXorf15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22357-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ELRG, Environmental lipopolysaccharide-responding gene protein, Factor inhibiting ATF4-mediated transcription, FIAT, Gamma taxilin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22357-1-PBS targets CXorf15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17936

Product name: Recombinant human CXorf15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 447-528 aa of BC101576

Sequence: ELNEKVEVLKEQVSIKAAIKAANRDLATPVMQPCTALDSHKELNTSSKRALGAHLEAEPKSQRSAVQKPPSTGSAPAIESVD

相同性解析による交差性が予測される生物種
Full Name chromosome X open reading frame 15
Calculated molecular weight 528 aa, 61 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC101576
Gene Symbol CXorf15
Gene ID (NCBI) 55787
RRIDAB_2879087
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9NUQ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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