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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCR7 Monoclonal antibody (60216-1-Ig)

    WB analysis using 60216-1-Ig (same clone as 60216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60216-1-Ig (CXCR7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IHC staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IHC staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human prostate cancer using 60216-1-Ig (same clone as 60216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of mouse spleen using 60216-1-Ig (same clone as 60216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

CXCR7 Monoclonal antibody, PBS Only

CXCR7 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60216-1-PBS
Clone No.4C3D7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ACKR3, Atypical chemokine receptor 3, Chemokine orphan receptor 1, CMKOR1, C-X-C chemokine receptor type 7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60216-1-PBS targets CXCR7 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14247

Product name: Recombinant human CXCR7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 273-362 aa of BC036661

Sequence: LLDIFSILHYIPFTCRLEHALFTALHVTQCLSLVHCCVNPVLYSFINRNYRYELMKAFIFKYSAKTGLTKLIDASRVSETEYSALEQSTK

相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 7
Calculated molecular weight 362 aa, 41 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC036661
Gene Symbol CXCR7
Gene ID (NCBI) 57007
RRIDAB_11182925
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25106
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).