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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using CXCR2 Polyclonal antibody (20634-1-AP)

    WB analysis of L02 using 20634-1-AP (same clone as 20634-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CXCR2 Polyclonal antibody (20634-1-AP)

    WB analysis of COLO 320 using 20634-1-AP (same clone as 20634-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CXCR2 Polyclonal antibody (20634-1-AP)

    IP experiment of HepG2 using 20634-1-AP (same clone as 20634-1-PBS)

    IP result of anti-CXCR2 (IP:20634-1-AP, 4ug; Detection:20634-1-AP 1:1000) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR2 Polyclonal antibody (20634-1-AP)

    IHC staining of human tonsillitis using 20634-1-AP (same clone as 20634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CXCR2 Polyclonal antibody (20634-1-AP)

    IHC staining of human tonsillitis using 20634-1-AP (same clone as 20634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CXCR2 Polyclonal antibody (20634-1-AP)

    IHC staining of human colon using 20634-1-AP (same clone as 20634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CXCR2 Polyclonal antibody (20634-1-AP)

    IHC staining of human colon using 20634-1-AP (same clone as 20634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CXCR2 Polyclonal antibody (20634-1-AP)

    IF Staining of mouse liver using 20634-1-AP (same clone as 20634-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CXCR2 antibody (20634-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CXCR2 Polyclonal antibody (20634-1-AP)

    IF Staining of HepG2 using 20634-1-AP (same clone as 20634-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CXCR2 antibody (20634-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20634-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CXCR2 Polyclonal antibody, PBS Only

CXCR2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 20634-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

IL8RB, CD182, CDw128b, CMKAR2, C-X-C chemokine receptor type 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20634-1-PBS targets CXCR2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR2 fusion protein Ag14670 相同性解析による交差性が予測される生物種
Full Name interleukin 8 receptor, beta
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC037961
Gene Symbol IL8RB
Gene ID (NCBI) 3579
RRIDAB_10693624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25025
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IL8RB, also named as CXCR2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, and CD182, belongs to the G-protein coupled receptor 1 family. It is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. It binds to IL-8 with high affinity. IL8RB also binds with high affinity to CXCL3, GRO/MGSA, and NAP-2.