CXCL8/IL-8 Polyclonal antibody

CXCL8/IL-8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17038-1-AP

Synonyms

C X C motif chemokine 8, CXCL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP/IL 8 protein I, GCP/IL 8 protein II, GCP/IL 8 protein III, GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1, IL 8, IL8, IL-8, interleukin 8, LYNAP, MDNCF



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman tonsillitis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit. CXCL8/IL-8 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17038-1-AP targets CXCL8/IL-8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL8/IL-8 fusion protein Ag10552 相同性解析による交差性が予測される生物種
Full Name interleukin 8
Calculated molecular weight 99 aa, 11 kDa
GenBank accession numberBC013615
Gene symbol IL8
Gene ID (NCBI) 3576
RRIDAB_2126217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Protocols

Product Specific Protocols
WB protocol for CXCL8/IL-8 antibody 17038-1-APDownload protocol
IHC protocol for CXCL8/IL-8 antibody 17038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Mol Cancer

RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.

Authors - Han-Hee Park
human

EMBO Rep

LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.

Authors - Xuedan Sun
humanIHC,IF

Cell Death Discov

Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.

Authors - Guojing Li
human

Cell Death Dis

Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression.

Authors - Kezhou Zhu
IHC

Int Immunopharmacol

Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis

Authors - Wei Wu
humanIHC

J Neurooncol

Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study.

Authors - Pedram Tabatabaei