Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98497-3-RR targets CXCL7/PPBP in FC (Intra) applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3405 Product name: Recombinant Rat CXCL7/PPBP protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 46-107 aa of NM_153721.1 Sequence: IELRCRCTNTLSGIPLNSISRVNVFRPGAHCDNVEVIATLKNGKEVCLDPTAPMIKKIVKKI 相同性解析による交差性が予測される生物種 |
| Full Name | pro-platelet basic protein (chemokine (C-X-C motif) ligand 7) |
| Calculated molecular weight | 12 kDa |
| GenBank accession number | NM_153721.1 |
| Gene Symbol | Ppbp |
| Gene ID (NCBI) | 246358 |
| RRID | AB_3746127 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99ME0 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CXCL7/PPBP antibody 98497-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |