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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIX/CXCL5 Polyclonal antibody (10809-1-AP)

    WB analysis using 10809-1-AP (same clone as 10809-1-PBS)

    Untreated, PMA treated A549 cells were subjected to SDS PAGE followed by western blot with 10809-1-AP (CXCL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using LIX/CXCL5 Polyclonal antibody (10809-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10809-1-AP (same clone as 10809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10809-1-AP (CXCL5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10809-1-PBS in a different storage buffer formulation.

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LIX/CXCL5 Polyclonal antibody, PBS Only

LIX/CXCL5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10809-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CXCL5, C X C motif chemokine 5, C-X-C motif chemokine 5, CXCL 5, CXCL-5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10809-1-PBS targets LIX/CXCL5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIX/CXCL5 fusion protein Ag1079 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 5
Calculated molecular weight 12 kDa
Observed molecular weight10-12 kDa
GenBank accession numberBC008376
Gene Symbol CXCL5
Gene ID (NCBI) 6374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42830
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chemokines are a class of pro-inflammatory cytokines that can recruit and activate chemotactic cells. CXCL5 is a member of the chemokine family binding CXCR2, a G-protein coupled receptor. CXCL5 is a chemokine that promotes tumor formation by triggering the migration of immune cells to tumors and promotes immmuno-suppressive characteristics of the tumor microenvironment. CXCL5 can also promote tumor cell metastasis and recruit vascular endothelial cells for angiogenesis.