Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29589-1-AP targets CXCL16 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30981 Product name: Recombinant human CXCL16 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-225 aa of BC017588 Sequence: ASEGASSDIHTPAQMLLSTLQSTQRPTLPVGSLSSDKELTRPNETTIHTAGHSLAAGPEAGENQKQPEKNAGPTARTSATV 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-X-C motif) ligand 16 |
| Calculated molecular weight | 273 aa, 30 kDa |
| Observed molecular weight | 28 kDa , 50-55 kDa |
| GenBank accession number | BC017588 |
| Gene Symbol | CXCL16 |
| Gene ID (NCBI) | 58191 |
| RRID | AB_3086142 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2A7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CXCL16 was discovered as a ligand for CXC chemokine receptor (CXCR) 6. CXCL16 is a membrane-bound chemokine that consists of four distinct domains: the chemokine domain, mucin-like domain, transmembrane domain and ad cytoplasmic. After cleavage, soluble CXCL16 acts as a chemoattractant for activated CD8 T cells, NKT cells and Th1-polarized T cells that express CXCR6. CXCL16 expression has been studied mainly in macrophages and dendritic cells. CXCL16 production by non-immune cells remains controversial. CXCL16 was expressed as two different forms: a 50 kDa form and a 28 kDa form, and the 50 kDa may be the glycosylated form (PMID: 17855433, 33552057). (PMID: 17855433, 33552057).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCL16 antibody 29589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |