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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CXCL10 Recombinant antibody (85307-2-RR)

    WB analysis of THP-1 using 85307-2-RR

    IFN gamma, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 85307-2-RR (CXCL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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CXCL10/IP-10 Recombinant antibody

CXCL10/IP-10 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85307-2-RR
Clone No.250120A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CXCL10, CXCL10/IP10, IP10, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inIFN gamma, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85307-2-RR targets CXCL10/IP-10 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXCL10/IP-10 fusion protein Eg2489 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 10
Calculated molecular weight 11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC010954
Gene Symbol CXCL10
Gene ID (NCBI) 3627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

Protocols

Product Specific Protocols
WB protocol for CXCL10/IP-10 antibody 85307-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols