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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CX3CR1 Monoclonal antibody (66915-1-Ig)

    WB analysis using 66915-1-Ig (same clone as 66915-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CX3CR1 Monoclonal antibody (66915-1-Ig)

    WB analysis of Jurkat using 66915-1-Ig (same clone as 66915-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CX3CR1 Monoclonal antibody (66915-1-Ig)

    IHC staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66915-1-Ig (CX3CR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CX3CR1 Monoclonal antibody (66915-1-Ig)

    IF Staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (66915-1-Ig, Clone: 3D3B8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

CX3CR1 Monoclonal antibody, PBS Only

CX3CR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66915-1-PBS
Clone No.3D3B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Beta chemokine receptor like 1, Beta chemokine receptor-like 1, C X3 C CKR 1, CCRL1, CMK BRL 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66915-1-PBS targets CX3CR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27698

Product name: Recombinant human CX3CR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 56-231 aa of BC028078

Sequence: LTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIK

相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) receptor 1
Calculated molecular weight 40 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC028078
Gene Symbol CX3CR1
Gene ID (NCBI) 1524
RRIDAB_2882242
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP49238
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CX3CR1, also known as GPR13, V28, and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed in microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as a coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

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