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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CX3CL1 Recombinant antibody (83738-5-RR)

    WB analysis using 83738-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83738-5-RR (Cx3cl1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CX3CL1 Recombinant antibody (83738-5-RR)

    IHC staining of mouse brain using 83738-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CX3CL1 Recombinant antibody (83738-5-RR)

    IHC staining of mouse brain using 83738-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83738-5-RR

    Affinity and Kinetic Characterization of 83738-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83738-5-RR against Mouse CX3CL1 were performed. The affinity constant is 0.744 nM.

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CX3CL1 Recombinant antibody

CX3CL1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83738-5-RR
Clone No.240738E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Scyd1, Cxc3, CX3C, ABCD 3, 240738E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse lung tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83738-5-RR targets CX3CL1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) ligand 1
Calculated molecular weight42 kDa
Observed molecular weight55-75 kDa
GenBank accession numberNM_009142.3
Gene Symbol Cx3cl1
Gene ID (NCBI) 20312
RRIDAB_3671337
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO35188
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).

Protocols

Product Specific Protocols
WB protocol for CX3CL1 antibody 83738-5-RRDownload protocol
IHC protocol for CX3CL1 antibody 83738-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols