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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using CX3CL1 Monoclonal antibody (60339-1-Ig)

    WB analysis of PC-3 using 60339-1-Ig (same clone as 60339-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60339-1-Ig (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CX3CL1 Monoclonal antibody (60339-1-Ig)

    IHC staining of human brain using 60339-1-Ig (same clone as 60339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CX3CL1 Monoclonal antibody (60339-1-Ig)

    IHC staining of human brain using 60339-1-Ig (same clone as 60339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CX3CL1 Monoclonal antibody (60339-1-Ig)

    IHC staining of human small intestine using 60339-1-Ig (same clone as 60339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CX3CL1 Monoclonal antibody (60339-1-Ig)

    IHC staining of human small intestine using 60339-1-Ig (same clone as 60339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60339-1-PBS in a different storage buffer formulation.

CX3CL1 Monoclonal antibody, PBS Only

CX3CL1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60339-1-PBS
Clone No.2F5D5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CX3C membrane-anchored chemokine, C3Xkine, C X3 C motif chemokine 1, ABCD 3, A-152E5.2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60339-1-PBS targets CX3CL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0161

Product name: Recombinant human CX3CL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 90-245 aa of BC001163

Sequence: DRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQ

相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) ligand 1
Calculated molecular weight 42 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001163
Gene Symbol CX3CL1
Gene ID (NCBI) 6376
RRIDAB_2881448
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP78423
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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