Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31700-1-AP targets CWF19L1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CWF19L1 fusion protein Ag36356 相同性解析による交差性が予測される生物種 |
| Full Name | CWF19-like 1, cell cycle control (S. pombe) |
| Observed molecular weight | 60-65 kDa |
| GenBank accession number | BC008746 |
| Gene Symbol | CWF19L1 |
| Gene ID (NCBI) | 55280 |
| RRID | AB_3670086 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q69YN2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CWF19L1 belongs to the CWF19 family. CWF19L1 includes a metallophosphatase (MMP) domain, a histidine triad motif (HIT) domain, and a C-terminal Cwf-J-C domain. CWF19L1 is believed to be involved in cell cycle control, endosomal trafficking, and mRNA processing. CWF19L1 is a rare cause of autosomal recessive cerebellar ataxias (ARCA) (PMID: 36357319, PMID: 36453471). CWF19L1 is expressed in many brain regions, including cerebellum, thalamus and occipital, parietal and temporal lobes. CWF19L1 is also expressed in the spinal cord (PMID: 25361784).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CWF19L1 antibody 31700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |