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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CWC25 Polyclonal antibody (24416-1-AP)

    WB analysis of HeLa using 24416-1-AP (same clone as 24416-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24416-1-AP (CWC25 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human kidney using 24416-1-AP (same clone as 24416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human kidney using 24416-1-AP (same clone as 24416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human cervical cancer using 24416-1-AP (same clone as 24416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human cervical cancer using 24416-1-AP (same clone as 24416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human cervical cancer using 24416-1-AP (same clone as 24416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human cervical cancer using 24416-1-AP (same clone as 24416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CWC25 Polyclonal antibody (24416-1-AP)

    IF Staining of HepG2 using 24416-1-AP (same clone as 24416-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CWC25 antibody (24416-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24416-1-PBS in a different storage buffer formulation.

CWC25 Polyclonal antibody, PBS Only

CWC25 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24416-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24416-1-PBS targets CWC25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17037

Product name: Recombinant human CCDC49 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 76-425 aa of BC008833

Sequence: GPGGMVNRDEYLLGRPIDKYVFEKMEEKEAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKKKKKEKKKKHKKHKHRSSSSDRSSSEDEHSAGRSQKKMANSSPVLSKVPGYGLQVRNSDRNQGLQGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKSTREAGSRDRRSRSLGRRSRSPRPSKLHNSKVNRRETGQTRSPSPKKEVYQRRHAPGYTRKLSAEELERKRQEMMENAKWREEERLNILKRHAKDEEREQRLEKLDSRDGKFIHRMKLESASTSSLEDRVKRNIYSLQRTSVALEKNFMKR

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 49
Calculated molecular weight 425 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC008833
Gene Symbol CCDC49
Gene ID (NCBI) 54883
RRIDAB_2879535
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXE8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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