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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUX2 Recombinant antibody (82933-1-RR)

    WB analysis using 82933-1-RR (same clone as 82933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82933-1-RR (CUX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using CUX2 Recombinant antibody (82933-1-RR)

    WB analysis of SH-SY5Y using 82933-1-RR (same clone as 82933-1-PBS)

    WB result of CUX2 antibody (82933-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUX2 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 82933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUX2 Recombinant antibody (82933-1-RR)

    IF Staining of HeLa using 82933-1-RR (same clone as 82933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUX2 Recombinant antibody (82933-1-RR)

    IF Staining of HeLa using 82933-1-RR (same clone as 82933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82933-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82933-1-RR

    Affinity and Kinetic Characterization of 82933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82933-1-RR against Human CUX2 were performed. The affinity constant is 1.02 nM.

  • Featured Product
  • KD/KO Validated

CUX2 Recombinant monoclonal antibody, PBS Only

CUX2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82933-1-PBS
Clone No.230235G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82933-1-PBS targets CUX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18809

Product name: Recombinant human CUX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-433 aa of BC151245

Sequence: RSLDDRLQPPSFDPSGQPRRDLHTSWKRNPELLSPKEQREGTSPAGPTLTEGSRLPGIPGKALLTETLLQRNEAEKQKGLQEVQITLAARLGEAEEKIKVLHSALKATQAELLELRRKYDEEAASKADEVGLIMTNLEKANQRAEAAQREVESLREQLASVNSSIRLACCSPQGPSGDKVNFTLCSGPRLEAALASKDREILRLLKDVQHLQSSLQELEEASANQIADLERQLTAKSEAIEKLEEKLQAQSDYEEIKTELSILKAMKLASSTCSLPQGMAKPEDSLLIAKEAFFPTQKFLLEKPSLLASPEEDPSEDDSIKDSL

相同性解析による交差性が予測される生物種
Full Name cut-like homeobox 2
Calculated molecular weight 1486 aa, 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberBC151245
Gene Symbol CUX2
Gene ID (NCBI) 23316
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO14529
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cut like homeobox 2 (CUX2) also named as, CDP2 or CUTL2, is a 1486 amino acid protein, which contains one homeobox DNA-binding domain and three CUT DNA-binding domains. CUX2 localizes in the nucleus and belongs to the CUT homeobox family. CUX2 may as a transcription factor be involved in neural specification.

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