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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis of human brain using 11733-1-AP (same clone as 11733-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis using 11733-1-AP (same clone as 11733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis of human liver using 11733-1-AP (same clone as 11733-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis of Jurkat using 11733-1-AP (same clone as 11733-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis of mouse liver using 11733-1-AP (same clone as 11733-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis of rat brain using 11733-1-AP (same clone as 11733-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    WB analysis of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IP experiment of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    IP result of anti-CUX1 (IP:11733-1-AP, 4ug; Detection:11733-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CUX1 Polyclonal antibody (11733-1-AP)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CUX1 Polyclonal antibody (11733-1-AP)

    IF Staining of SH-SY5Y using 11733-1-AP (same clone as 11733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CUX1 antibody (11733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cortex using CUX1 Polyclonal antibody (11733-1-AP)

    IF Staining of mouse cortex using 11733-1-AP (same clone as 11733-1-PBS)

    IF result of the antibody CUX1 (11733-1-AP, 1:300) with mouse cortex, Data from Dr. Lee O. Vaasjo Muñoz, University of Chicago. . This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CUX1 Polyclonal antibody, PBS Only

CUX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11733-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

COY1, Clox, CDP1, CDP/Cut, CDP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11733-1-PBS targets CUX1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2335

Product name: Recombinant human CUX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-341 aa of BC025422

Sequence: MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLS

相同性解析による交差性が予測される生物種
Full Name cut-like homeobox 1
Calculated molecular weight 676 aa, 77 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC025422
Gene Symbol CUX1
Gene ID (NCBI) 1523
RRIDAB_2086995
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13948
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).

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