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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CUTC Polyclonal antibody (15030-1-AP)

    WB analysis of HeLa using 15030-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15030-1-AP (CUTC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CUTC Polyclonal antibody (15030-1-AP)

    WB analysis of mouse skeletal muscle using 15030-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15030-1-AP (CUTC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CUTC Polyclonal antibody (15030-1-AP)

    IHC staining of human endometrial cancer using 15030-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15030-1-AP (CUTC antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CUTC Polyclonal antibody (15030-1-AP)

    IHC staining of human endometrial cancer using 15030-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15030-1-AP (CUTC antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CUTC Polyclonal antibody (15030-1-AP)

    IHC staining of human pancreas using 15030-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15030-1-AP (CUTC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CUTC Polyclonal antibody (15030-1-AP)

    IHC staining of human pancreas using 15030-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15030-1-AP (CUTC antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUTC Polyclonal antibody (15030-1-AP)

    IF Staining of HeLa using 15030-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUTC antibody (15030-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CUTC Polyclonal antibody

CUTC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15030-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CGI-32

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue
Positive IHC detected inhuman endometrial cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15030-1-AP targets CUTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2126

Product name: Recombinant human CUTC protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-273 aa of BC021105

Sequence: MKRQGASSERKRARIPSGKAGAANGFLMEVCVDSVESAVNAERGGADRIELCSGLSEGGTTPSMGVLQVVKQSVQIPVFVMIRPRGGDFLYSDREIEVMKADIRLAKLYGADGLVFGALTEDGHIDKELCMSLMAICRPLPVTFHRAFDMVHDPMAALETLLTLGFERVLTSGCDSSALEGLPLIKRLIEQAKGRIVVMPGGGITDRNLQRILEGSGATEFHCSARSTRDSGMKFRNSSVAMGASLSCSEYSLKVTDVTKVRTLNAIAKNILV

相同性解析による交差性が予測される生物種
Full Name cutC copper transporter homolog (E. coli)
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC021105
Gene Symbol CUTC
Gene ID (NCBI) 51076
RRIDAB_2086984
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CUTC antibody 15030-1-APDownload protocol
IHC protocol for CUTC antibody 15030-1-APDownload protocol
WB protocol for CUTC antibody 15030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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