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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL4A Monoclonal antibody (66038-1-Ig)

    WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    WB analysis of pig brain using 66038-1-Ig (same clone as 66038-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CUL4A Monoclonal antibody (66038-1-Ig)

    WB analysis of HeLa using 66038-1-Ig (same clone as 66038-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CUL4A Monoclonal antibody (66038-1-Ig)

    IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

    IP result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human breast cancer using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human breast cancer using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CUL4A Monoclonal antibody (66038-1-Ig)

    IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CUL4A Monoclonal antibody, PBS Only

CUL4A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66038-1-PBS
Clone No.1A7F12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

1A7F12, CUL 4A, CUL-4A, cullin 4A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66038-1-PBS targets CUL4A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

Tested Reactivity human, mouse, rat, pig, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18089

Product name: Recombinant human CUL4A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 460-759 aa of BC008308

Sequence: RLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA

相同性解析による交差性が予測される生物種
Full Name cullin 4A
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa, 88 kDa
GenBank accession numberBC008308
Gene Symbol CUL4A
Gene ID (NCBI) 8451
RRIDAB_11042446
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13619
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

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