• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CUL3 Polyclonal antibody (28087-1-AP)

    WB analysis using 28087-1-AP (same clone as 28087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CUL3 Polyclonal antibody (28087-1-AP)

    WB analysis using 28087-1-AP (same clone as 28087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CUL3 Polyclonal antibody (28087-1-AP)

    IP experiment of mouse testis using 28087-1-AP (same clone as 28087-1-PBS)

    IP result of anti-CUL3 (IP:28087-1-AP, 4ug; Detection:28087-1-AP 1:600) with mouse testis tissue lysate 1680 ug. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL3 Polyclonal antibody (28087-1-AP)

    IHC staining of human prostate cancer using 28087-1-AP (same clone as 28087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL3 Polyclonal antibody (28087-1-AP)

    IHC staining of human prostate cancer using 28087-1-AP (same clone as 28087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

CUL3 Polyclonal antibody, PBS Only

CUL3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 28087-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CUL-3, Cullin-3, KIAA0617

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28087-1-PBS targets CUL3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28004

Product name: Recombinant human CUL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC039598

Sequence: MSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFE

相同性解析による交差性が予測される生物種
Full Name cullin 3
Calculated molecular weight 89 kDa
Observed molecular weight80-88 kDa
GenBank accession numberBC039598
Gene Symbol CUL3
Gene ID (NCBI) 8452
RRIDAB_2918147
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13618
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

||
New chat

Able™

正在加载，请稍候...