Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | mouse pancreas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16416-1-AP targets Chymotrypsin C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9572 Product name: Recombinant human CTRC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-268 aa of BC015118 Sequence: MLGITVLAALLACASSCGVPSFPPNLSARVVGGEDARPHSWPWQISLQYLKNDTWRHTCGGTLIASNFVLTAAHCISNTRTYRVAVGKNNLEVEDEEGSLFVGVDTIHVHKRWNALLLRNDIALIKLAEHVELSDTIQVACLPEKDSLLPKDYPCYVTGWGRLWTNGPIADKLQQGLQPVVDHATCSRIDWWGFRVKKTMVCAGGDGVISACNGDSGGPLNCQLENGSWEVFGIVSFGSRRGCNTRKKPVVYTRVSAYIDWINEKMQL 相同性解析による交差性が予測される生物種 |
| Full Name | chymotrypsin C (caldecrin) |
| Calculated molecular weight | 268 aa, 29 kDa |
| Observed molecular weight | 30 kDa, 35 kDa |
| GenBank accession number | BC015118 |
| Gene Symbol | CTRC |
| Gene ID (NCBI) | 11330 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99895 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chymotrypsin C (chymotrypsin C) is a serine protease of digestive tract encoded by human CTRC gene. Secreted by pancreatic acinar cells, it is not only involved in the protein degradation of food, but also the "protector" of the pancreas, which can prevent the premature activation of trypsin in the pancreas by degrading trypsinogen. If the mutation of CTRC gene leads to the loss of protein's function, it will destroy the original protective degradation process of trypsinogen, and lead to the proliferation of abnormally activated trypsin in the pancreas, thus significantly increasing the risk of hereditary or idiopathic chronic pancreatitis (PMID: 35594281). The molecular weight of the protein is 30 kDa(PMID: 21828354), and there is N- glycosylation modification, and the modified molecular weight is 35kDa. The same protein may present different glycosylation forms in two species (PMID: 34118474).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Chymotrypsin C antibody 16416-1-AP | Download protocol |
| WB protocol for Chymotrypsin C antibody 16416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |