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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of mouse pancreas using 24939-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of rat pancreas using 24939-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of rat pancreas using 24939-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of mouse pancreas using 24939-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

CTRB1 Polyclonal antibody

CTRB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24939-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

chymotrypsinogen B1, CTRB, CTRB1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24939-1-AP targets CTRB1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8604

Product name: Recombinant human CTRB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-263 aa of BC005385

Sequence: SGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVNNDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANKTPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLVCQKDGAWTLVGIVSWGSDTCSTSSPGVYARVTKLIPWVQKILAAN

相同性解析による交差性が予測される生物種
Full Name chymotrypsinogen B1
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005385
Gene Symbol CTRB1
Gene ID (NCBI) 1504
RRIDAB_2879809
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP17538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CTRB1 antibody 24939-1-APDownload protocol
WB protocol for CTRB1 antibody 24939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB，IF

Biol Open

Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.

Authors - Timothy L Hostelley
View Article
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