Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Raji using 21264-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21264-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21264-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21264-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 21264-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 21264-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 21264-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 21264-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 21264-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 21264-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 21264-1-AP
Immunofluorescent analysis of Hela cells, using CTR9 antibody 21264-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CTR9 Polyclonal antibody

CTR9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21264-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CTR9, KIAA0155, p150, p150TSP, SH2 domain binding protein 1, SH2BP1, TSBP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, HEK-293 cells, HeLa cells, L02 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below

Product Information

21264-1-AP targets CTR9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15690 Product name: Recombinant human CTR9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-338 aa of BC058914 Sequence: MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARIDGNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLLEGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVKLNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFFFKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASS 相同性解析による交差性が予測される生物種
Full Name	Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight	1173 aa, 134 kDa
Observed molecular weight	130-150 kDa
GenBank accession number	BC058914
Gene Symbol	CTR9
Gene ID (NCBI)	9646
RRID	AB_10734585
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6PD62
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CTR9 antibody 21264-1-AP	Download protocol
IHC protocol for CTR9 antibody 21264-1-AP	Download protocol
WB protocol for CTR9 antibody 21264-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Autophagy The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy. Authors - Liangde Zheng View Article
mouse	WB	Front Pharmacol Tetrandrine Prevents Bone Loss in Ovariectomized Mice by Inhibiting RANKL-Induced Osteoclastogenesis. Authors - Zeyuan Zhong View Article
mouse	WB	Cell Death Dis UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer. Authors - Gang Xiang View Article
human	WB	Cell SARS-CoV-2 variants evolve convergent strategies to remodel the host response Authors - Mehdi Bouhaddou View Article
human	IHC	Eur J Histochem MicroRNA-17-3p is upregulated in psoriasis and regulates keratinocyte hyperproliferation and pro-inflammatory cytokine secretion by targeting CTR9 Authors - Qingwen Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Raji using 21264-1-AP

WB analysis of mouse lung using 21264-1-AP

WB analysis of HeLa using 21264-1-AP

WB analysis of HEK-293 using 21264-1-AP

WB analysis of L02 using 21264-1-AP

WB analysis of mouse testis using 21264-1-AP

IHC staining of human breast cancer using 21264-1-AP

IHC staining of human breast cancer using 21264-1-AP

IHC staining of human colon cancer using 21264-1-AP

IHC staining of human colon cancer using 21264-1-AP

IF Staining of HeLa using 21264-1-AP

CTR9 Polyclonal antibody

CTR9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Tetrandrine Prevents Bone Loss in Ovariectomized Mice by Inhibiting RANKL-Induced Osteoclastogenesis.

UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.

SARS-CoV-2 variants evolve convergent strategies to remodel the host response

MicroRNA-17-3p is upregulated in psoriasis and regulates keratinocyte hyperproliferation and pro-inflammatory cytokine secretion by targeting CTR9