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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTPS2 Polyclonal antibody

CTPS2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12852-1-AP

Synonyms

CTP synthase 2, CTP synthase II, CTP synthetase 2, CTPS2, UTP ammonia ligase 2

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 12852-1-AP

    WB analysis of mouse testis using 12852-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 12852-1-AP

    WB analysis of mouse testis using 12852-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12852-1-AP

    WB analysis of HeLa using 12852-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human adrenal gland using 12852-1-AP

    WB analysis of human adrenal gland using 12852-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12852-1-AP

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12852-1-AP

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12852-1-AP

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 12852-1-AP

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 12852-1-AP

    IHC staining of human colon using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 12852-1-AP

    IHC staining of human colon using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, human adrenal gland tissue
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

12852-1-AP targets CTPS2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTPS2 fusion protein Ag3490 相同性解析による交差性が予測される生物種
Full Name CTP synthase II
Calculated molecular weight 586 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034986
Gene symbol CTPS2
Gene ID (NCBI) 56474
RRIDAB_10639520
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

Protocols

Product Specific Protocols
WB protocol for CTPS2 antibody 12852-1-APDownload protocol
IHC protocol for CTPS2 antibody 12852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int Rev Cell Mol Biol

Molecular cell biology and immunobiology of mammalian rod/ring structures.

Authors - Wendy C Carcamo
View Article
humanIF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise
View Article
humanWB

J Genet Genomics

Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.

Authors - Gerson Dierley Keppeke
View Article