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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    WB analysis of mouse testis using 12852-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    WB analysis of mouse testis using 12852-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTPS2 Polyclonal antibody (12852-1-AP)

    WB analysis of HeLa using 12852-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human adrenal gland tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    WB analysis of human adrenal gland using 12852-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CTPS2 Polyclonal antibody (12852-1-AP)

    IP experiment of HeLa using 12852-1-AP

    IP result of anti-CTPS2 (IP:12852-1-AP, 4ug; Detection:12852-1-AP 1:300) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human colon using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12852-1-AP (CTPS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human colon using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12852-1-AP (CTPS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human prostate cancer using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human colon using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CTPS2 Polyclonal antibody (12852-1-AP)

    IHC staining of human colon using 12852-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

CTPS2 Polyclonal antibody

CTPS2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12852-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

UTP--ammonia ligase 2, UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, human adrenal gland tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12852-1-AP targets CTPS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3490

Product name: Recombinant human CTPS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-373 aa of BC034986

Sequence: CGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAVQEWVMNQAKVPVDGNKEEPQICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHVSLVPQLSATGEQKTKPTQNSVRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASNLLFKWRNMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETEDPVKFHEAWQKLCKADGILVPGGF

相同性解析による交差性が予測される生物種
Full Name CTP synthase II
Calculated molecular weight 586 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034986
Gene Symbol CTPS2
Gene ID (NCBI) 56474
RRIDAB_10639520
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

Protocols

Product Specific Protocols
IHC protocol for CTPS2 antibody 12852-1-APDownload protocol
IP protocol for CTPS2 antibody 12852-1-APDownload protocol
WB protocol for CTPS2 antibody 12852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int Rev Cell Mol Biol

Molecular cell biology and immunobiology of mammalian rod/ring structures.

Authors - Wendy C Carcamo
View Article
humanIF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise
View Article
humanWB

J Genet Genomics

Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.

Authors - Gerson Dierley Keppeke
View Article
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