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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTP synthase Recombinant monoclonal antibody (86455-1-RR)

    WB analysis using 86455-1-RR (same clone as 86455-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86455-1-RR (CTPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86455-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CTP synthase Recombinant monoclonal antibody (86455-1-RR)

    WB analysis of HeLa using 86455-1-RR (same clone as 86455-1-PBS)

    WB result of CTP synthase antibody (86455-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CTP synthase transfected HeLa cells. This data was developed using the same antibody clone with 86455-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CTP synthase Recombinant monoclonal antibody (86455-1-RR)

    IF Staining of HeLa using 86455-1-RR (same clone as 86455-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CTP synthase antibody (86455-1-RR, Clone: 251260F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86455-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CTP synthase Recombinant monoclonal antibody (86455-1-RR)

    IF Staining of NIH/3T3 using 86455-1-RR (same clone as 86455-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CTP synthase antibody (86455-1-RR, Clone: 251260F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86455-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86455-1-RR

    Affinity and Kinetic Characterization of 86455-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86455-1-RR against Human CTP synthase were performed. The affinity constant is below 1 pM.

CTP synthase Recombinant monoclonal antibody, PBS Only

CTP synthase Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86455-1-PBS
Clone No.251260F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CTPS, CTPS 1, CTPS1, EC:6.3.4.2, UTP--ammonia ligase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86455-1-PBS targets CTP synthase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8707

Product name: Recombinant human CTPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 242-591 aa of BC009408

Sequence: ICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRIGSSSPDSEITELKFPSINHD

相同性解析による交差性が予測される生物種
Full Name CTP synthase
Calculated molecular weight 591 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC009408
Gene Symbol CTPS
Gene ID (NCBI) 1503
RRIDAB_3744896
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17812
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CTP synthase (CTPS) is a ubiquitous, cytosolic glutamine amidotransferase that catalyzes the ATP-dependent conversion of uridine-5′-triphosphate (UTP) to cytidine-5′-triphosphate (CTP) - the final, rate-limiting step in the de novo pyrimidine biosynthetic pathway.

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