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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis using 16534-1-AP (same clone as 16534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16534-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CTHRC1 Polyclonal antibody (16534-1-AP)

    WB analysis using 16534-1-AP (same clone as 16534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16534-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A375 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    IP experiment of A375 using 16534-1-AP (same clone as 16534-1-PBS)

    IP result of anti-CTHRC1 (IP:16534-1-AP, 3ug; Detection:16534-1-AP 1:500) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 16534-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    IF Staining of A375 using 16534-1-AP (same clone as 16534-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using CTHRC1 antibody (16534-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16534-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    IF Staining of HepG2 using 16534-1-AP (same clone as 16534-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CTHRC1 antibody (16534-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16534-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A375 cells using CTHRC1 Polyclonal antibody (16534-1-AP)

    FC experiment of A375 using 16534-1-AP (same clone as 16534-1-PBS)

    1x10^6 A375 cells were intracellularly stained with 0.13 ug CTHRC1 Polyclonal antibody (16534-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16534-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CTHRC1 Polyclonal antibody, PBS Only

CTHRC1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16534-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

collagen triple helix repeat containing 1, Collagen triple helix repeat-containing protein 1, Protein NMTC1, UNQ762/PRO1550

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16534-1-PBS targets CTHRC1 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9812

Product name: Recombinant human CTHRC1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-243 aa of BC014245

Sequence: MRPQGPAASPQRLRGLLLLLLLQLPAPSSASEIPKGKQKAQLRQREVVDLYNGMCLQGPAGVPGRDGSPGANGIPGTPGIPGRDGFKGEKGECLRESFEESWTPNYKQCSWSSLNYGIDLGKIAECTFTKMRSNSALRVLFSGSLRLKCRNACCQRWYFTFNGAECSGPLPIEAIIYLDQGSPEMNSTINIHRTSSVEGLCEGIGAGLVDVAIWVGTCSDYPKGDASTGWNSVSRIIIEELPK

相同性解析による交差性が予測される生物種
Full Name collagen triple helix repeat containing 1
Calculated molecular weight 243 aa, 26 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC014245
Gene Symbol CTHRC1
Gene ID (NCBI) 115908
RRIDAB_2087511
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CG8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).

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