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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of MCF-7 using 17532-1-AP (same clone as 17532-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of mouse eye using 17532-1-AP (same clone as 17532-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of HepG2 using 17532-1-AP (same clone as 17532-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of Y79 using 17532-1-AP (same clone as 17532-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    WB analysis of human brain using 17532-1-AP (same clone as 17532-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IP experiment of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    IP result of anti-CTDSPL2 (IP:17532-1-AP, 4ug; Detection:17532-1-AP 1:500) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CTDSPL2 Polyclonal antibody (17532-1-AP)

    IF Staining of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTDSPL2 antibody (17532-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

CTDSPL2 Polyclonal antibody, PBS Only

CTDSPL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17532-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CTD small phosphatase-like protein 2, CTDSP like 2, CTDSP-like 2, EC:3.1.3.-, HSPC058

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17532-1-PBS targets CTDSPL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11581

Product name: Recombinant human CTDSPL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 114-466 aa of BC035744

Sequence: EMTNQHVKQNGKLEDNPSSGSPPRTTLLGTIFSPVFNFFSPANKNGTSGSDSPGQAVEAEEIVKQLDMEQVDEITTSTTTSTNGAAYSNQAVQVRPSLNNGLEEAEETVNRDIPPLTAPVTPDSGYSSAHAEATYEEDWEVFDPYYFIKHVPPLTEEQLNRKPALPLKTRSTPEFSLVLDLDETLVHCSLNELEDAALTFPVLFQDVIYQVYVRLRPFFREFLERMSQMYEIILFTASKKVYADKLLNILDPKKQLVRHRLFREHCVCVQGNYIKDLNILGRDLSKTIIIDNSPQAFAYQLSNGIPIESWFMDKNDNELLKLIPFLEKLVELNEDVRPHIRDRFRLHDLLPPD

相同性解析による交差性が予測される生物種
Full Name CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
Calculated molecular weight 466 aa, 53 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC035744
Gene Symbol CTDSPL2
Gene ID (NCBI) 51496
RRIDAB_2087204
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05D32
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.

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