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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using CTDSP2 Polyclonal antibody (11116-1-AP)

    WB analysis of human kidney using 11116-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CTDSP2 Polyclonal antibody (11116-1-AP)

    WB analysis of K-562 using 11116-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CTDSP2 Polyclonal antibody (11116-1-AP)

    WB analysis of HepG2 using 11116-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CTDSP2 Polyclonal antibody (11116-1-AP)

    IHC staining of human pancreas cancer using 11116-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11116-1-AP (CTDSP2 antibody) at dilution of 1:50 (under 10x lens).

CTDSP2 Polyclonal antibody

CTDSP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11116-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CTDSP2, NIF2, NLI interacting factor 2, OS4, Protein OS 4, PSR2, SCP2, Small CTD phosphatase 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman kidney tissue, HepG2 cells, K-562 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11116-1-AP targets CTDSP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1601

Product name: Recombinant human CTDSP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC065920

Sequence: MEHGSIITQARREDALVLTKQGLVSKSSPKKPRGRNIFKALFCCFRAQHVGQSSSSTELAAYKEEANTIAKSDLLQCLQYQFYQIPGTCLLPEVTEEDQGRICVVIDLDETLVHSSFKPINNADFIVPIEIEGTTHQVYVLKRPYVDEFLRRMGELFECVLFTASLAKYADPVTDLLDRCGVFRARLFRESCVFHQGCYVKDLSRLGRDLRKTLILDNSPASYIFHPENAVPVQSWFDDMADTELLNLIPIFEELSGAEDVYTSLGQLRAP

相同性解析による交差性が予測される生物種
Full Name CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC065920
Gene Symbol CTDSP2
Gene ID (NCBI) 10106
RRIDAB_2087086
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14595
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTDSPs are C-class phosphatases that act on Ser 5 of the Pol II C-terminal domain (CTD) to decrease transcription [PMID:15681389]. CTDSP enzymatic activity is required for REST function, and knockdown of CTDSP induces the neuronal differentiation of mouse embryonic carcinoma P19 cells. CTDSP2 has been shown to enhance TGF-β signaling by the dephosphorylation of SMAD proteins and attenuates androgen receptor-mediated transcription [PMID:16724108]. It negatively regulates RNA polymerase II transcription by controlling the transition from initiation/capping to processive transcript elongation. It was recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells[PMID: 12721286].

Protocols

Product Specific Protocols
IHC protocol for CTDSP2 antibody 11116-1-APDownload protocol
WB protocol for CTDSP2 antibody 11116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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