Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66995-1-Ig (same clone as 66995-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 66995-1-Ig (same clone as 66995-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 66995-1-Ig (same clone as 66995-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 66995-1-Ig (same clone as 66995-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66995-1-Ig (same clone as 66995-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.
×
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

CTBP1 Monoclonal antibody, PBS Only

CTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 66995-1-PBS

Clone No.1C4B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1C4B2, BARS, CTBP, C-terminal-binding protein 1, EC:1.1.1.-

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66995-1-PBS targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28567 Product name: Recombinant human CTBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-440 aa of BC011655 Sequence: MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL 相同性解析による交差性が予測される生物種
Full Name	C-terminal binding protein 1
Calculated molecular weight	48 kDa
Observed molecular weight	45-48 kDa
GenBank accession number	BC011655
Gene Symbol	CTBP1
Gene ID (NCBI)	1487
RRID	AB_2882312
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q13363
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

WB analysis of HEK-293 using 66995-1-Ig (same clone as 66995-1-PBS)

WB analysis of A549 using 66995-1-Ig (same clone as 66995-1-PBS)

WB analysis of HL-60 using 66995-1-Ig (same clone as 66995-1-PBS)

WB analysis of HSC-T6 using 66995-1-Ig (same clone as 66995-1-PBS)

IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)