Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10972-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 10972-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10972-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10972-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10972-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10972-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10972-1-AP
IP result of anti-CTBP1 (IP:10972-1-AP, 3ug; Detection:10972-1-AP 1:200) with mouse brain tissue lysate 4000ug.
IHC staining of human prostate cancer using 10972-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10972-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10972-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10972-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (10972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 10972-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (10972-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 10972-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CTBP1 (10972-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

CTBP1 Polyclonal antibody

CTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10972-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

BARS, CTBP, C-terminal-binding protein 1, EC:1.1.1.-

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human prostate cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 9 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

10972-1-AP targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1425 Product name: Recombinant human CTBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-440 aa of BC011655 Sequence: MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL 相同性解析による交差性が予測される生物種
Full Name	C-terminal binding protein 1
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC011655
Gene Symbol	CTBP1
Gene ID (NCBI)	1487
RRID	AB_2086628
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13363
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Protocols

Product Specific Protocols
IF protocol for CTBP1 antibody 10972-1-AP	Download protocol
IHC protocol for CTBP1 antibody 10972-1-AP	Download protocol
IP protocol for CTBP1 antibody 10972-1-AP	Download protocol
WB protocol for CTBP1 antibody 10972-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression. Authors - Yongqing Liu View Article
human	WB	Nat Commun ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin. Authors - Lifang Li KD Validated View Article
human	WB,IF	Oncogene RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer. Authors - Yang Yu View Article
human	WB	Mol Oncol Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma. Authors - Xuejie Min View Article
human	WB	Mol Carcinog Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma. Authors - Kirthana Prabhakar KD Validated View Article
mouse	WB	Front Physiol Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice Authors - Yuxiu Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10972-1-AP

WB analysis using 10972-1-AP

WB analysis of mouse thymus using 10972-1-AP

WB analysis of mouse skeletal muscle using 10972-1-AP

WB analysis of mouse brain using 10972-1-AP

WB analysis of mouse thymus using 10972-1-AP

IP experiment of mouse brain using 10972-1-AP

IHC staining of human prostate cancer using 10972-1-AP

IHC staining of human prostate cancer using 10972-1-AP

IHC staining of mouse brain using 10972-1-AP

IF Staining of HepG2 using 10972-1-AP

IF Staining of HepG2 using 10972-1-AP

FC experiment of HepG2 using 10972-1-AP

CTBP1 Polyclonal antibody

CTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.

ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.

RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.

Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.

Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.

Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice