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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using MARK3 Polyclonal antibody (12932-1-AP)

    WB analysis of human brain using 12932-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12932-1-AP (MARK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MARK3 Polyclonal antibody (12932-1-AP)

    WB analysis of human brain using 12932-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12932-1-AP (MARK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MARK3 Polyclonal antibody (12932-1-AP)

    WB analysis of PC-3 using 12932-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12932-1-AP (MARK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MARK3 Polyclonal antibody (12932-1-AP)

    IP experiment of mouse brain using 12932-1-AP

    IP result of anti-MARK3 (IP:12932-1-AP, 3ug; Detection:12932-1-AP 1:500) with mouse brain tissue lysate 4000ug.

MARK3 Polyclonal antibody

MARK3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12932-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, PC-3 cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12932-1-AP targets MARK3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3805

Product name: Recombinant human CTAK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC024773

Sequence: MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQK

相同性解析による交差性が予測される生物種
Full Name MAP/microtubule affinity-regulating kinase 3
Calculated molecular weight 78 kDa, 80 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC024773
Gene Symbol MARK3
Gene ID (NCBI) 4140
RRIDAB_10638613
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP27448
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MARK3 antibody 12932-1-APDownload protocol
IP protocol for MARK3 antibody 12932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols