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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTAGE5 Recombinant antibody (83017-2-RR)

    WB analysis using 83017-2-RR (same clone as 83017-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83017-2-RR (CTAGE5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83017-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTAGE5 Recombinant antibody (83017-2-RR)

    IF Staining of HepG2 using 83017-2-RR (same clone as 83017-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTAGE5 antibody (83017-2-RR, Clone: 230262F2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83017-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CTAGE5 Recombinant antibody (83017-2-RR)

    FC experiment of HepG2 using 83017-2-RR (same clone as 83017-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CTAGE5 Recombinant antibody (83017-2-RR, Clone:230262F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83017-2-PBS in a different storage buffer formulation.

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CTAGE5 Recombinant antibody, PBS Only

CTAGE5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83017-2-PBS
Clone No.230262F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor, cTAGE family member 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83017-2-PBS targets CTAGE5 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CTAGE family, member 5
Calculated molecular weight 92 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_005930
Gene Symbol CTAGE5
Gene ID (NCBI) 4253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PC5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241).