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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTAGE5 Polyclonal antibody

CTAGE5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55279-1-AP

Synonyms

cTAGE family member 5, CTAGE family, member 5, CTAGE5, MEA11, MEA6, MGEA, MGEA11, MGEA6, MIA2, Protein cTAGE 5

Validation Data Gallery

Applications:
  • WB analysis of human testis using 55279-1-AP

    WB analysis of human testis using 55279-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 55279-1-AP

    WB analysis of human testis using 55279-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 55279-1-AP

    WB analysis of human brain using 55279-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 55279-1-AP

    WB analysis of human brain using 55279-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 55279-1-AP

    WB analysis of human testis using 55279-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 55279-1-AP

    IHC staining of human prostate cancer using 55279-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 55279-1-AP

    IHC staining of human prostate cancer using 55279-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman testis tissue, human brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55279-1-AP targets CTAGE5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CTAGE family, member 5
Calculated molecular weight 92 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberNM_005930
Gene symbol CTAGE5
Gene ID (NCBI) 4253
RRIDAB_11183044
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241).This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.

Protocols

Product Specific Protocols
WB protocol for CTAGE5 antibody 55279-1-APDownload protocol
IHC protocol for CTAGE5 antibody 55279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols