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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CTAGE5 Polyclonal antibody (55279-1-AP)

    WB analysis of HepG2 using 55279-1-AP

    WB result of CTAGE5 antibody (55279-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CTAGE5 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using CTAGE5 Polyclonal antibody (55279-1-AP)

    WB analysis using 55279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CTAGE5 Polyclonal antibody (55279-1-AP)

    WB analysis of human brain using 55279-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTAGE5 Polyclonal antibody (55279-1-AP)

    IHC staining of human prostate cancer using 55279-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTAGE5 Polyclonal antibody (55279-1-AP)

    IHC staining of human prostate cancer using 55279-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

CTAGE5 Polyclonal antibody

CTAGE5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55279-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MEA6, MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, human brain tissue, PC-3 cells, human testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55279-1-AP targets CTAGE5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CTAGE family, member 5
Calculated molecular weight 92 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberNM_005930
Gene Symbol CTAGE5
Gene ID (NCBI) 4253
RRIDAB_11183044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.

Protocols

Product Specific Protocols
WB protocol for CTAGE5 antibody 55279-1-APDownload protocol
IHC protocol for CTAGE5 antibody 55279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols