Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells | 
| Positive IP detected in | HeLa cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below | 
Product Information
13267-1-AP targets CTAGE1 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human | 
| Cited Reactivity | human | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag3948 Product name: Recombinant human CTAGE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 466-780 aa of BC031065 Sequence: DNWSAAWTAERNLNDLRKENAHNRQKLTEIEFKIKLLEKDPYGLDVPNTAFGRQHSPYGPSPLGWPSSETRASLYPPTLLEGPLRLSPLLPRGGGRGSRGPGNPPDHQITKERGESSCDRLTDPHRAPSDAGPLAPPWEQDYRMMFPPPGQSYPDSALPPQRQDRFYSNCARLSGPAELRSFNMPSLDKMDGSMPSEMESSRNDTKDNLGNLKVPDSSLPAENEATGPGFVPPPLAPIRGLLFPVDTRGPFIRRGPPFPPPPPGTVFGASPDYFSPRDVPGPPRAPFAMRNVYLPRGFLPYRPPRPAFFPPAPTF相同性解析による交差性が予測される生物種 | 
| Full Name | cutaneous T-cell lymphoma-associated antigen 1 | 
| Calculated molecular weight | 754 aa, 89 kDa | 
| Observed molecular weight | 86-120 kDa | 
| GenBank accession number | BC031065 | 
| Gene Symbol | CTAGE1 | 
| Gene ID (NCBI) | 64693 | 
| RRID | AB_2086621 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | Q96RT6 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CTAGE1 antibody 13267-1-AP | Download protocol | 
| WB protocol for CTAGE1 antibody 13267-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. | ||
| Clin Cancer Res Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients. | 


