Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 24290-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 24290-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 24290-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 24290-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 24290-1-AP
IP result of anti-CSTF3 (IP:24290-1-AP, 4ug; Detection:24290-1-AP 1:500) with A431 cells lysate 1200ug.
IHC staining of human stomach cancer using 24290-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24290-1-AP (CSTF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CSTF3 Polyclonal antibody

CSTF3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 24290-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CF-1 77 kDa subunit, CSTF 77, CstF-77

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, K-562 cells, HEK-293 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

24290-1-AP targets CSTF3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17930 Product name: Recombinant human CSTF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-103 aa of BC059948 Sequence: MSGDGATEQAAEYVPEKVKKAEKKLEENPYDLDAWSILIREAQNQPIDKARKTYERLVAQFPSSGRFWKLYIEAEVTILFYFFLYQYCSIHCSDRKQVRNIAN 相同性解析による交差性が予測される生物種
Full Name	cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
Calculated molecular weight	717 aa, 83 kDa
Observed molecular weight	77-83 kDa
GenBank accession number	BC059948
Gene Symbol	CSTF3
Gene ID (NCBI)	1479
RRID	AB_2879475
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12996
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for CSTF3 antibody 24290-1-AP	Download protocol
IP protocol for CSTF3 antibody 24290-1-AP	Download protocol
WB protocol for CSTF3 antibody 24290-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Death Dis CSTF3 contributes to platinum resistance in ovarian cancer through alternative polyadenylation of lncRNA NEAT1 and generating the short isoform NEAT1_1 Authors - Xin Luo View Article
human,mouse	WB,IF,IP	iScience Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability Authors - Ziqian Min KD Validated View Article
human	WB	Comput Struct Biotechnol J Alternative polyadenylation regulates the translation of metabolic and inflammation-related proteins in adipose tissue of gestational diabetes mellitus Authors - Bingnan Chen View Article

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Validation Data Gallery

WB analysis using 24290-1-AP

WB analysis of K-562 using 24290-1-AP

WB analysis of A431 using 24290-1-AP

WB analysis of A431 using 24290-1-AP

IP experiment of A431 using 24290-1-AP

IHC staining of human stomach cancer using 24290-1-AP