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  • Featured Product
  • KD/KO Validated

CST6 Polyclonal antibody

CST6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17076-1-AP

Synonyms

CST6, Cystatin 6, Cystatin E, cystatin E/M, Cystatin M

Validation Data Gallery

Applications:
  • IHC staining of human breast cancer using 17076-1-AP

    IHC staining of human breast cancer using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 17076-1-AP

    IHC staining of human breast cancer using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 17076-1-AP

    IHC staining of mouse skin using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 17076-1-AP

    IHC staining of mouse skin using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 17076-1-AP

    IHC staining of mouse skin using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 17076-1-AP

    IHC staining of mouse skin using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 17076-1-AP

    IHC staining of mouse heart using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse heart using 17076-1-AP

    IHC staining of mouse heart using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse liver using 17076-1-AP

    IHC staining of mouse liver using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse liver using 17076-1-AP

    IHC staining of mouse liver using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 17076-1-AP

    IHC staining of human breast cancer using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 17076-1-AP

    IHC staining of human breast cancer using 17076-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman breast cancer tissue, mouse skin tissue, mouse heart tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17076-1-AP is CST6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CST6 fusion protein Ag10965 相同性解析による交差性が予測される生物種
Full Name cystatin E/M
Calculated molecular weight 149 aa, 17 kDa
GenBank accession numberBC031334
Gene symbol CST6
Gene ID (NCBI) 1474
RRIDAB_2878345
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CST6 antibody 17076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis

Authors - Xiaoxun Li
  • KD Validated
View Article
humanWB

J Proteome Res

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Authors - Yong Zhou
View Article
humanIHC

Int J Gen Med

Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery

Authors - Xiaopeng Zhu
View Article