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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cystatin SN/CST1 Recombinant antibody (85403-1-RR)

    WB analysis using 85403-1-RR (same clone as 85403-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 85403-1-RR (CST1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85403-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01926-3

    Sandwich ELISA standard curve of MP01926-3

    Sandwich ELISA standard curve of MP01926-3, Human Cystatin SN/CST1 Recombinant Matched Antibody Pair - PBS only. 85403-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8862. 85403-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-10 ng/mL

  • Sample test of MP01926-3

    Sample test of MP01926-3

    Urine of seven individual healthy human donors was measured. The human Cystatin SN/CST1 concentration of detected samples was determined to be 0.70 ng/mL with a range of 0.16 - 2.31 ng/mL.

  • Sample test of MP01926-3

    Sample test of MP01926-3

    Serum of eight individual healthy human donors was measured. The human Cystatin SN/CST1 concentration of detected samples was determined to be 5.27 ng/mL with a range of 2.95 - 7.40 ng/mL.

  • Cytometric bead array standard curve of MP01926-2

    Cytometric bead array standard curve of MP01926-2

    Cytometric bead array standard curve of MP01926-2, Cystatin SN/CST1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85403-1-PBS. Detection antibody: 85403-2-PBS. Standard: Ag8862. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01926-1

    Cytometric bead array standard curve of MP01926-1

    Cytometric bead array standard curve of MP01926-1, Cystatin SN/CST1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85403-3-PBS. Detection antibody: 85403-1-PBS. Standard: Ag8862. Range: 0.313-40 ng/mL.

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Cystatin SN/CST1 Recombinant antibody, PBS Only (Capture/Detector)

Cystatin SN/CST1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85403-1-PBS
Clone No.242888B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CST1, Cystain SA I, Cystain-SA-I, Cystatin 1, cystatin SN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85403-1-PBS targets Cystatin SN/CST1 as part of a matched antibody pair:

MP01926-1: 85403-3-PBS capture and 85403-1-PBS detection (validated in Cytometric bead array)

MP01926-2: 85403-1-PBS capture and 85403-2-PBS detection (validated in Cytometric bead array)

MP01926-3: 85403-4-PBS capture and 85403-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cystatin SN/CST1 fusion protein Ag8862 相同性解析による交差性が予測される生物種
Full Name cystatin SN
Calculated molecular weight 141 aa, 16 kDa
Observed molecular weight14-16 kDa
GenBank accession numberBC021225
Gene Symbol CST1
Gene ID (NCBI) 1469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01037
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.