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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSRP2 Polyclonal antibody (10892-2-AP)

    WB analysis using 10892-2-AP (same clone as 10892-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CSRP2 Polyclonal antibody (10892-2-AP)

    WB analysis using 10892-2-AP (same clone as 10892-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using CSRP2 Polyclonal antibody (10892-2-AP)

    IP experiment of mouse liver using 10892-2-AP (same clone as 10892-2-PBS)

    IP result of anti-CSRP2 (IP:10892-2-AP, 4ug; Detection:10892-2-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CSRP2 Polyclonal antibody (10892-2-AP)

    IHC staining of mouse kidney using 10892-2-AP (same clone as 10892-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10892-2-AP (CSRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CSRP2 Polyclonal antibody, PBS Only

CSRP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10892-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CRP2, Cysteine and glycine-rich protein 2, Cysteine rich protein 2, Cysteine-rich protein 2, LMO5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10892-2-PBS targets CSRP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1338

Product name: Recombinant human CSRP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-193 aa of BC000992

Sequence: MPVWGGGNKCGACGRTVYHAEEVQCDGRSFHRCCFLCMVCRKNLDSTTVAIHDEEIYCKSCYGKKYGPKGYGYGQGAGTLNMDRGERLGIKPESVQPHRPTTNPNTSKFAQKYGGAEKCSRCGDSVYAAEKIIGAGKPWHKNCFRCAKCGKSLESTTLTEKEGEIYCKGCYAKNFGPKGFGYGQGAGALVHAQ

相同性解析による交差性が予測される生物種
Full Name cysteine and glycine-rich protein 2
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000992
Gene Symbol CSRP2
Gene ID (NCBI) 1466
RRIDAB_2087738
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16527
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CSRP2 (Cysteine and glycine-rich protein 2) is a member of the LIM-only family of cysteine-rich proteins and contains two LIM domains, which has been recently implicated in the progression and metastasis of a variety of cancers. CSRP2 is functionally linked to the activity of TGF-b1 in hepatic stellate cells (HSC), derivatives thereof, and in differentiated VSMC.CSRP2 knockdown signifcantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells (PMID: 33042270). CSRP2 is a short (21 kDa) two LIM domain-containing protein, which is upregulated in invasive breast cancer cells, and localizes along the protrusive actin core of invadopodium1 (PMID: 29976963, 16735029).

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