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Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using CSRP1 Polyclonal antibody (13432-1-AP)

    WB analysis of human stomach using 13432-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse stomach tissue using CSRP1 Polyclonal antibody (13432-1-AP)

    IP experiment of mouse stomach using 13432-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:300.

  • Immunohistochemistry (IHC) staining of human colon tissue using CSRP1 Polyclonal antibody (13432-1-AP)

    IHC staining of human colon using 13432-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CSRP1 Polyclonal antibody (13432-1-AP)

    IHC staining of human colon using 13432-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CSRP1 Polyclonal antibody (13432-1-AP)

    IHC staining of human lung cancer using 13432-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CSRP1 Polyclonal antibody (13432-1-AP)

    IHC staining of human lung cancer using 13432-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 40x lens).

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CSRP1 Polyclonal antibody

CSRP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13432-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

CRP, CRP1, CSRP, CYRP, Cysteine and glycine-rich protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman stomach tissue
Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman normal colon, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13432-1-AP targets CSRP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSRP1 fusion protein Ag4236 相同性解析による交差性が予測される生物種
Full Name cysteine and glycine-rich protein 1
Calculated molecular weight 193 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC032493
Gene Symbol CSRP1
Gene ID (NCBI) 1465
RRIDAB_2230180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSRP1 (Cysteine and glycine-rich protein 1), designated initially as CRP1, constitutes a cysteine-rich protein derived from placental sources and exhibits a response profile analogous to c-myc. CSRP1 has been recognized as a tumor suppressor in several types of cancers including colorectal cancer, and cholangiocarcinoma (PMID: 37113556). Abnormal expression of CSRP1 was reported within several malignancies such as prostate cancer and acute myeloid leukemia (PMID: 38813484, 40198006).

Protocols

Product Specific Protocols
WB protocol for CSRP1 antibody 13432-1-APDownload protocol
IHC protocol for CSRP1 antibody 13432-1-APDownload protocol
IP protocol for CSRP1 antibody 13432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Med Rep

Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes.

Authors - Hui Ren
View Article