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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK2B Polyclonal antibody (20234-1-AP)

    WB analysis using 20234-1-AP (same clone as 20234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CSNK2B Polyclonal antibody (20234-1-AP)

    WB analysis of HepG2 using 20234-1-AP (same clone as 20234-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CSNK2B Polyclonal antibody (20234-1-AP)

    WB analysis of MCF-7 using 20234-1-AP (same clone as 20234-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using CSNK2B Polyclonal antibody (20234-1-AP)

    WB analysis of A431 using 20234-1-AP (same clone as 20234-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSNK2B Polyclonal antibody (20234-1-AP)

    IHC staining of human breast cancer using 20234-1-AP (same clone as 20234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSNK2B Polyclonal antibody (20234-1-AP)

    IHC staining of human breast cancer using 20234-1-AP (same clone as 20234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CSNK2B Polyclonal antibody (20234-1-AP)

    FC experiment of HeLa using 20234-1-AP (same clone as 20234-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CSNK2B-specific (20234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CSNK2B Polyclonal antibody, PBS Only

CSNK2B Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 20234-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

CSNK2B-specific, Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20234-1-PBS targets CSNK2B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name casein kinase 2, beta polypeptide
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_001320
Gene Symbol CSNK2B
Gene ID (NCBI) 1460
RRIDAB_10733246
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP67870
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.

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