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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00953-1

    Sandwich ELISA standard curve of MP00953-1

    Sandwich ELISA standard curve of MP00953-1, Human CSF2RA/CD116 Recombinant Matched Antibody Pair - PBS only. 98066-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02335. 84023-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00953-1

    Sample test of MP00953-1

    Serum of six individual healthy human donors was measured. The CSF2RA/CD116 concentration of detected samples was determined to be 2.0 ng/mL with a range of 0.9-4.7 ng/mL.

  • Sample test of MP00953-1

    Sample test of MP00953-1

    The mean CSF2RA/CD116 concentration was determined to be 84.1 ng/mL in THP-1 cell extract based on a 4.0 mg/mL extract load and 59.0 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibod (98066-1-RR)

    FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control. Cells were then stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC). Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibod (98066-1-RR)

    FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibod (98066-1-RR)

    FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), and 0.25 ug APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00953-1

    Cytometric bead array standard curve of MP00953-1

    Cytometric bead array standard curve of MP00953-1, CSF2RA/CD116 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98066-1-PBS. Detection antibody: 84023-1-PBS. Standard: RP02335. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 98066-1-RR

    Affinity and Kinetic Characterization of 98066-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98066-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.52 nM.

Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody, PBS Only

CSF2RA/CD116 Uni-rAb® Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98066-1-PBS
Clone No.241266B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD116, 241266B11, GMCSFR, GM CSF R alpha, CSF2RA

Formulation:  PBS Only
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98066-1-PBS targets CSF2RA/CD116 in FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Calculated molecular weight 46 kDa
GenBank accession numberBC002635
Gene Symbol CD116
Gene ID (NCBI) 1438
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP15509
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

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