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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using CSF1R/CD115 Recombinant antibody (84919-4-RR)

    WB analysis of RAW 264.7 using 84919-4-RR (same clone as 84919-4-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84919-4-RR (CSF1R/CD115 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CSF1R/CD115 Recombinant antibody (84919-4-RR)

    IF Staining of RAW 264.7 using 84919-4-RR (same clone as 84919-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CSF1R/CD115 antibody (84919-4-RR, Clone: 242213E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84919-4-PBS in a different storage buffer formulation.

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Mouse CSF1R/CD115 Recombinant antibody, PBS Only

CSF1R/CD115 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84919-4-PBS
Clone No.242213E1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Csf1r, Csfmr, CSF-1 receptor, CSF 1R, CD115

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84919-4-PBS targets CSF1R/CD115 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 1 receptor
Calculated molecular weight109 kDa
Observed molecular weight165 kDa
GenBank accession numberNM_001037859.2
Gene Symbol CSF1R/CD115
Gene ID (NCBI) 12978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09581
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).