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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSF1R/CD115 Recombinant monoclonal antibody (84477-6-RR)

    WB analysis using 84477-6-RR (same clone as 84477-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84477-6-RR (CSF1R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84477-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CSF1R/CD115 Recombinant monoclonal antibody (84477-6-RR)

    IF Staining of THP-1 using 84477-6-RR (same clone as 84477-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF1R antibody (84477-6-RR, Clone: 241675D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84477-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84477-6-RR

    Affinity and Kinetic Characterization of 84477-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84477-6-RR against Human CSF1R were performed. The affinity constant is 0.775 nM.

CSF1R/CD115 Recombinant monoclonal antibody, PBS Only

CSF1R/CD115 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84477-6-PBS
Clone No.241675D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CSF1R, 241675D4, C FMS, CD115, CSF 1 R

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84477-6-PBS targets CSF1R/CD115 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 1 receptor
Calculated molecular weight 972 aa, 108 kDa
Observed molecular weight160 kDa
GenBank accession numberBC047521
Gene Symbol CSF1R/CD115
Gene ID (NCBI) 1436
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP07333
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

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