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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis of HeLa using 22219-1-AP (same clone as 22219-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW264.7 using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis of RAW264.7 using 22219-1-AP (same clone as 22219-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CSE1L Polyclonal antibody (22219-1-AP)

    WB analysis of MCF-7 using 22219-1-AP (same clone as 22219-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CSE1L Polyclonal antibody (22219-1-AP)

    IP experiment of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)

    IP result of anti-CSE1L (IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CSE1L Polyclonal antibody (22219-1-AP)

    IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CSE1L Polyclonal antibody (22219-1-AP)

    IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CSE1L Polyclonal antibody (22219-1-AP)

    IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CSE1L Polyclonal antibody (22219-1-AP)

    IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CSE1L Polyclonal antibody, PBS Only

CSE1L Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 22219-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22219-1-PBS targets CSE1L in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17556

Product name: Recombinant human CSE1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 627-971 aa of BC109313

Sequence: RITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL

相同性解析による交差性が予測される生物種
Full Name CSE1 chromosome segregation 1-like (yeast)
Calculated molecular weight 971 aa, 110 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC109313
Gene Symbol CSE1L
Gene ID (NCBI) 1434
RRIDAB_10950892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP55060
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.

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