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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSDA Polyclonal antibody

CSDA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27785-1-AP

Synonyms

cold shock domain protein A, CSDA, CSDA1, DBPA, DNA binding protein A, YBX3, ZONAB

Validation Data Gallery

Applications:
  • WB analysis using 27785-1-AP

    WB analysis using 27785-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27785-1-AP (CSDA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27785-1-AP

    IHC staining of mouse heart using 27785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 27785-1-AP

    IHC staining of mouse skeletal muscle using 27785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, Y79 cells, mouse liver tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27785-1-AP targets CSDA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSDA fusion protein Ag26839 相同性解析による交差性が予測される生物種
Full Name cold shock domain protein A
Calculated molecular weight 40 kDa
Observed molecular weight60-68 kDa
GenBank accession numberBC008801
Gene symbol CSDA
Gene ID (NCBI) 8531
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cold shock domain protein A (CSDA) is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE).

Protocols

Product Specific Protocols
WB protocol for CSDA antibody 27785-1-APDownload protocol
IHC protocol for CSDA antibody 27785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols